Extended Data Fig. 2: Effects of maternal rhythm disruption on neonatal MDSCs.
(a-c) WT pups cross-fostered by Bmal1fl/flWAPcre (n = 8) or Bmal1fl/fl (n = 8) mother were subjected to NEC induction. (a) The survival rate of pups. (b) Representative H&E staining of intestine and the Inflammation scores. scale bars, 100 μm (n = 3-6). (c) The expression of the pro-inflammation genes Il6 and Il1β in intestine was determined by qRT–PCR (n = 3-6). (d-e) The frequencies and absolute numbers of indicated immune cell types in spleen of 7-day-old pups were analysed by flow cytometry (n = 5). (f) The immunosuppressive function of splenic M-MDSC of pups (n = 3-5). (g) The immunosuppressive activity of neonatal PMN-MDSCs from control and CRD groups at different time points (n = 3/time point). (h) The mRNA expression of clock genes Cry2, Per3 and Nr1d2 in PMN-MDSCs from pups were determined by qRT–PCR (n = 3). Data are representative of two independent experiments. Mean ± SEM were shown. Log-rank (Mantel-Cox) test for a. Two-tailed unpaired Student’s t test for b, c, e, f and two-way ANOVA followed by Bonferroni’s multiple comparisons test for g. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, exact P values are provided in the source data.
