Extended Data Fig. 5: Levels of mitochondrial transcripts and mtDNA in different tissues of IMT-treated mice.
a-f, Levels of representative mitochondrial transcripts and mtDNA were measured in tissues of mice on chow diet or HFD treated with vehicle or IMT compound for four weeks. Levels of mtDNA in liver after four weeks of IMT treatment. n = 12 mice per group (a). The mtDNA transcript (b) and mtDNA (c) levels in eWAT. n = 10 mice per group. d, Representative western blot analyses of OXPHOS protein levels in eWAT after four weeks of vehicle or IMT treatment. Subunits of complex I (NDUFB8), complex II (SDHA and SDHB), complex IV (MTCOX1), and complex V (ATP5A) were analysed. VDAC was used as loading control. A representative image of n = 3 independent experiments is shown. The mtDNA transcript, n = 10 mice per group (e) and mtDNA, n = 6 mice per group (f) levels in skeletal muscle. The mtDNA transcript levels in heart (g) and brown adipose tissue (BAT, h). n = 6 mice per group. All data are presented as mean ± SEM. a, e, Statistical significance was assessed by the Mann–Whitney U-test. b, c, f-h, Statistical significance was assessed by a two-way ANOVA with Tukey’s test for multiple comparisons. P values are shown in the figure.
