Extended Data Fig. 1: Full DMS dataset.
From: Systematic analysis of NDUFAF6 in complex I assembly and mitochondrial disease
Heatmap representation of the DMS data. The residue position is labeled along the horizontal axis (missing residues in the dataset denoted by a black triangle), the amino acid substitution is labeled on the vertical axis. The color of the rectangles represents the fitness score of the variant. Gray dots mark the wild-type amino acid at each position. Additional annotations to aid interpretation are shown above and below the fitness score heatmaps. Conservation at each position (calculated using ConSurf) is shown using the ConSurf color scale. Predicted alpha-helical secondary structure based on the AlphaFold AF6 model is shown as a thick, grey line. Percent solvent-accessible surface area (% SASA) at each residue position, calculated from the AlphaFold AF6 model is shown as a bar graph.
