Extended Data Fig. 7: KL cells require SHMT for antioxidant defenses.
a, Abundance of HA-TPNOX and HA-mitoTPNOX in cells used in Fig. 4m and n. Vinculin was used as a loading control. b and c, Effect of G6PD (left), ME1 (middle), IDH1 (right) silencing on SHMT silencing-induced loss of viability in H2122 cells (b) and H1373 cells (c)(n = 3). d, Abundance of G6PD, ME1, IDH1, SHMT1 and 2 in cells used in Fig. 4o, and p. Vinculin was used as a loading control. e and f, Abundance of G6PD, ME1, IDH1, SHMT1 and 2 in cells used in Supplementary Fig. 7b (e) and c (f). Vinculin was used as a loading control. g and h, Effect of G6PDi (G6PD inhibitor, left), ME1i (ME1 inhibitor, middle), GSK321 (IDH1 inhibitor, right) on SHMT inhibition-induced viability loss in H460 cells (g) and H1373 cells (h)(n = 3). i and j, Effect of G6PDi (left), ME1i (middle), GSK321 (right) on SHMT inhibition-induced alteration of NADP+/NADPH ratio in H460 cells (i) and H1373 cells (j)(n = 3). k, Abundance of NADK2 used in Fig. 4l. Vinculin was used as a loading control. Data are the mean ± s.d. of independent cultures indicated in each panel. Statistical significance was assessed using a one-way ANOVA (b, c, g to j). In b and c, *p < 0.05 compared to EV-siCtrl; #p < 0.05 compared to EV-siSHMT1 + 2; $p < 0.05 compared to EV-siG6PD, ME1 or IDH1. In g to j, *p < 0.05 compared to DMSO; #p < 0.05 compared to SHIN2; $p < 0.05 compared to G6PDi, ME1i or GSK321. All experiments were repeated at least twice or more.
