Extended Data Fig. 3: Remyelination following cuprizone treatment requires mitochondrial complex III function in microglia.
(a) Representative MTR map of MRI images from RISP-cHET and RISP-cKO mice. White arrow in top right segment identifies the corpus callosum. (b) Axial section of MTR map with corpus callosum (green) and ventricle (red). (c) 3D rendering of corpus callosum and ventricle drawings used to quantify voxel intensity. (d) Odc-1 normalized mRNA counts from RISP-cHET and RISP-cKO microglia isolated from mice from two separate groups: from RISP-cHET and RISP-cKO microglia isolated from mice from two separate groups: 2 weeks into remyelination phase following cuprizone treatment RISP-cHET n = 4 (M = 2, F = 2), RISP-cKO n = 6 (M = 4, F = 2), aged 1-year RISP-cHET n = 9 (M = 7, F = 2) and RISP-cKO n = 10 (M = 7, F = 3), 1-year-old microglia data in this Fig is the same as Fig. 2. (e) Individual overlayed and individual fluorescent fields from Fig. 3b. In d a one-way ANOVA (followed by Tukey’s honest significant difference post-hoc test for multiple comparisons) was performed.
