Extended Data Fig. 6: Kinetic analysis of human PSAT1, GOT2, and GPT2.
From: The unique catalytic properties of PSAT1 mediate metabolic adaptation to glutamine blockade
a, Representative Lineweaver–Burk plot of the inverse initial velocity (1/V0) as a function of the inverse of the glutamate concentration (1/[S]) for PSAT1. b, Representative Lineweaver–Burk plot of the inverse initial velocity (1/V0) as a function of the inverse of the glutamate concentration (1/[S]) for GOT2. c, Representative Lineweaver–Burk plot of the inverse initial velocity (1/V0) as a function of the inverse of the glutamate concentration (1/[S]) for GPT2. d, Representative Lineweaver–Burk plot of the inverse initial velocity (1/V0) as a function of the inverse of the 3-PHP concentration (1/[S]) for PSAT1. PSAT1, phosphoserine aminotransferase 1, GOT2, glutamic-oxaloacetic transaminase 2; GPT2, glutamic–pyruvic transaminase 2; 3-PHP, 3-phosphohydroxypyruvate; Km, Michaelis constant; V0, initial velocity, [S], substrate concentration.
