Extended Data Fig. 8: Hepatocyte expression of WT mouse LXRα normalizes bodyweight, organ weights and serum biochemistry in Western diet-fed LXRαW441R/W441R mice.
10 male and 12 female 10–12 week old C57BL/6 J LXRαW441R/W441R mice were randomized to receive a tail vein injection with 1x10-11 GC of AAV8 expressing either LXRα and GFP (LXR) or just GFP (GFP) under the control of the hepatocyte-specific Thyroxine binding globulin (TBG) promoter (see methods) and a week later they were placed on western diet. A: Body weight curves of mice in each group on a western diet, N(GFP) = 5 male, 6 female mice, N(LXR) = 5 male, 6 female mice. B-E: Tissues weights of indicated organs at necropsy F-G: Serum cholesterol measurements after 8 weeks on Western diet. Throughout, the red bars and symbols represent mice treated with LXRα expressing virus, green represents mice injected with control GFP-expressing virus, grey indicate non-littermate 10-week old male wild-type mice not injected with virus as a comparison (N = 5). Repeated measures data (bodyweight) was analysed with a mixed-effects model with post hoc between group comparisons undertaken with the Holm-Šídak test with sex included in the model as a co-variate. All other data presented are analysed with a Two-way ANOVA with post hoc Holm-Šídak testing. Height of the bars represents mean ± SEM. For all biochemistry and organ weight data presented N(GFP) = 4 male, 6 female mice, N(LXR) = 4 male, 6 female mice.
