Extended Data Fig. 2: Effects of T1D on islet hormone contents and insulin secretion.
a-c, Islet insulin (a), glucagon (b) and (c) somatostatin contents in young ND ( < 7 weeks old; n = 18 groups of 20 islets from 6 mice, black) and adult (n = 13 experiments with islets from 5 mice, >12 weeks old) ND (grey) and T1D NOD mice (n = 7 experiments with islets from 5 mice; red). ****p < 0.0001 vs young ND NOD islets; †††p = 0.0005, ††††p < 0.0001 vs adult ND NOD mice (one-way ANOVA with Tukey post hoc test). d, Insulin secretion at 1 and 10 mM glucose in young ND (n = 6 experiments with islets from 6 mice), adult ND (n = 13 experiments with islets from 5 mice) and adult T1D NOD mice (n = 7 experiments with islets from 5 mice). **p = 0.009 and ****p = 9.4 × 10−7 vs 1 mM glucose of the same category. ††††p = 7.6 × 10−5 (Adult ND) and ††††p = 2.6 × 10−7 (Adult T1D) vs 10 mM glucose in young NOD mice. Two-sided Student’s t-test. e, Circulating somatostatin in adult ND (n = 8) and T1D NOD mice (n = 5). *p = 0.0035 vs ND. Two-sided Student’s t-test. In dot plots, rectangles and error bars behind data points represent mean values ± S.E.M.
