Extended Data Fig. 5: Impact of T1D on islet urocortin-3 contents and tolbutamide-induced somatostatin secretion.
a, Urocortin-3 (ucn3) contents of islets from C57BL/6 J, young ND NOD, adult ND and adult T1D NOD mice. ***p = 0.004 vs young ND islets; ††p = 0.0036 vs adult ND islets (n = 5-6 experiments with islets from 4–6 mice). Urocortin-3 content in C57BL/6 J islets is also significantly higher than that in adult ND (p = 0.0093) and T1D NOD (p < 0.0001) mouse islets. Statistical significances were evaluated using one-way ANOVA with Tukey post hoc test. b, Effects of tolbutamide on somatostatin secretion in isolated islets from young ND and adult T1D mice. **p = 0.006 and ****p = 5.4 × 10−7 vs no tolbutamide in the respective group of islets (n = 4–6 experiments with islets from 4 mice for ND and T1D mice), one-sided Student’s t-test. c, Quantitative PCR of Gjd2 expression in islets cultured with CC (n = 4 using islets from 6 mice). Data are normalised to Gjd2 expression in islets incubated without CC. *p = 0.0489 vs control, two-sided Student’s t-test. d, Violin plot of average scaled expression of Gjd2 from single-cell RNA sequencing data of δ-cells from wildtype (n = 298 cells; 2 samples; C57BL/6 J, black) and adult ND NOD (n = 1048 cells; 10 samples; red) mice. logFC = −0.16; ****p = 4.41 × 10−6, Wilcoxon Rank Sum test. e, Somatostatin secretion at 1 mM glucose in islets of wildtype (C57BL/6 J, black) and adult ND NOD (red) mice. ****p = 4.5 × 10−25 vs C57BL/6 J islets (n = 31 and 14 experiments using 4 mice for each group), two-sided Student’s t-test. In dot plots, rectangles and error bars behind data points represent mean values ± S.E.M.
