Extended Data Fig. 1: Systemic Tm6sf2 deficiency triggers MASH in mice.
(a) Experimental schematic of systematic Tm6sf2 KO and wildtype control mice fed with NC for 4 months; (b, c) Representative images of Tm6sf2 immunohistochemistry (b), and Tm6sf2 protein expression in liver tissues (c) of systematic Tm6sf2 KO and wildtype mice (n = 5 per group); (d-f) Representative images of Oil Red O and H&E staining with histological scoring (d), hepatic triglyceride and lipid peroxidation (e), and serum ALT level (f) in Tm6sf2 KO (n = 9) and wildtype mice (n = 5); (g-i) Experimental schematic and representative images of H&E staining with histological scoring (g), hepatic lipid peroxidation (h), and serum ALT level (i) of systematic Tm6sf2 KO and wildtype control mice fed with CD-HFD for 2 months (n = 8 per group). Results are presented as mean ± s.d. Statistical significance was determined by two-tailed Student’s t test (e, g-i) and Mann-Whitney U test (d and f).
