Extended Data Fig. 5: LC3B-interacting regions (LIRs) of NUFIP1 is irrelevant to its function on DDR regulation.
From: NUFIP1 integrates amino acid sensing and DNA damage response to maintain the intestinal homeostasis
a, Western blot of p-ATR, p-CHK1 and γH2A.X in HEK293T cells. 2 mM HU was added to cells with or without amino acids starvation. n = 3 independent experiments. b, Schematic representation of AAV-infected Nufip1(Δ/Δ) iIEC mice. Mice was fed with 6% protein diets and DDR was induced by 5-FU treatment. c, Proteins were isolated from colon tissue of mice in (b) and examined with indicated antibodies. Representative results were from three independent mice. d, The representative IHC images of p-ATR and γH2A.X in colon tissues from mice in (b). n = 3 mice. e, Quantification of IHC of p-ATR and γH2A.X in colonic crypts from mice in (b). n = 61 crypts. 3 independent experiments. Scale bar, 50 µm. The data were represented as mean±s.d. Statistical comparisons were made using one-way ANOVA test (a) or Kolmogorov-Smirnov test (e).
