Fig. 3: Effect of sensor levels on Gal1p and Gal3p sensing.
From: The galactokinase enzyme of yeast senses metabolic flux to stabilize galactose pathway regulation
a,b, Top, model prediction of the effect of a flux sensor (a) and concentration sensor (b) titration on signalling. Bottom, schematic overview of the responses to sensor level increases. c,d, Relationship between Gal1p (c) and Gal3p (d) levels and GAL signalling at different external galactose concentrations in the gal1Δ gal3Δ TetO7pr-GAL1-mScarlet-I (c) or gal1Δ gal3Δ TetO7pr-GAL3-mScarlet-I (d) strains. GAL signalling and sensor levels were determined and analysed as described in Fig. 1d. See Supplementary Note 3 for the quantitative definition of the regime-separating lines in a–d. See Extended Data Fig. 7 a comparison of replicate experiments.
