Fig. 4: Flux sensing and stability of GAL signalling.
From: The galactokinase enzyme of yeast senses metabolic flux to stabilize galactose pathway regulation
a–c, Single-cell time courses of GAL1pr-Ubi-Y-mNeonGreen strains with WT regulation (a), gal1Δ::GAL1pr-SpGAL1 (b) or gal3Δ (c) in a gal80Δ::CYC1pr-GAL80 background. For each genotype, 50 single-cell time courses were randomly selected from the population of cells whose GAL signalling was in the top 20% after 10 h in a medium with 4 mM glucose and 1 mM galactose (a and b) or 4 mM galactose (c). See Extended Data Fig. 9d for the replicate experiments.
