Fig. 2: Distinct glutamatergic dorsal hippocampal ensembles for fat and sugar.
a, Schematic of the FosTRAP approach comparing tdTomato labeling with Fos labeling in response to IG nutrient infusions. b, Representative images of the dDG in FosTRAP mice following IG infusion of fat or sugar (tdTomato, magenta, top), and 2 weeks later in the same mouse after IG infusion of fat (Fos, cyan; middle). Colocalization of neurons responding to both stimuli (white; bottom) along with a higher magnification image (center). c, Quantification of the overlap between neurons responding to repeated fat infusions (fat/fat) compared with infusions of different macronutrients (sugar/fat) in the dHPC. n = 5 mice per group, unpaired two-tailed t-test. d, Images of the dDG showing GABA mRNA expression (cyan) and tdTomato-positive neurons (magenta) in FosTRAP mice following IG infusion of sugar or fat. e, Proportion of tdTomato+ neurons colocalized with GABA mRNA. f, Images of the dDG showing vGLUT1 mRNA expression (cyan) and tdTomato-positive neurons (magenta) in FosTRAP mice following IG infusion of sugar or fat. g, Proportion of tdTomato+ neurons colocalized with vGLUT1 mRNA. Data are presented as mean ± s.e.m. ***P < 0.001. All images are representative of independent experiments from 5 animals. Scale bars, 100 µm. Elements of a were created in Biorender.
