Fig. 6: Sugar-responsive dHPC neurons promote food intake.

a, Schematic of experimental paradigm for ad libitum 45% high-fat, high-sugar (HFHS) diet intake from BioDAQ in dHPC^Sugar mice expressing either the control or hM3Dq virus. b, Cumulative hourly food intake in response to saline or CNO (n = 6 mice per group, two-way within-subjects ANOVA with Holm–Sidak post hoc analysis). c, Schematic of experimental paradigm for ad libitum 60% high-fat (HF) diet intake from BioDAQ in dHPC^Fat mice expressing either the control or hM3Dq virus. d, Cumulative hourly food intake in response to saline or CNO (n = 6, two-way within-subjects ANOVA with Holm–Sidak post hoc analysis). e–g dHPC^Sugar mice expressing control or caspase virus maintained on HFHS diet for 10 days in BioDAQ. e, Ten-day total HFHS diet intake (n = 10 for Sugar^Con, n = 9 for Sugar^Casp3, unpaired two-tailed t-test). f, Average cumulative HFHS diet intake in dark phase (n = 11 for Sugar^Con, n = 12 for Sugar^Casp3, two-way ANOVA with Holm–Sidak post hoc analysis). g, Average cumulative HFHS diet intake in light phase (n = 11 for Sugar^Con, n = 12 for Sugar^Casp3, two-way ANOVA with Holm–Sidak post hoc analysis). h,i, Body weight gain (h) and fat mass accumulation (i) of control mice on HFHS diet for 4 weeks (n = 9 mice per group, RM one-way ANOVA with Holm–Sidak post hoc analysis). j,k, Body weight gain (j) and fat mass accumulation (k) of dHPC^Sugar mice with caspase ablation on HFHS diet for 4 weeks (n = 9 mice per group, RM one-way ANOVA with Holm–Sidak post hoc analysis). l,n, dHPC^Fat mice expressing control or caspase virus maintained on HF diet for 10 days in BioDAQ. l, Ten-day total HF diet intake (n = 7 mice per group, unpaired two-tailed t-test). m, Average cumulative HF diet intake in dark phase (n = 7 mice per group, two-way ANOVA with Holm–Sidak post hoc analysis). n, Average cumulative HF diet intake in light phase (n = 7 mice per group, two-way ANOVA with Holm–Sidak post hoc analysis). o,p, Body weight gain (o) and fat mass accumulation (p) of control mice on HF diet for 4 weeks (n = 9 mice per group, RM one-way ANOVA with Holm–Sidak post hoc analysis). q,r, Body weight gain (q) and fat mass accumulation (r) of dHPC^Fat mice with caspase ablation on HF diet for 4 weeks (n = 7 mice per group, RM One-way ANOVA with Holm–Sidak post hoc analysis). Data are presented as mean ± s.e.m. Elements of a and c were created in Biorender.

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