Extended Data Fig. 4: TFE3 knockout perturbs NADH/NAD+ ratio selectively in tRCC.
a, NAD+ or NADH levels in ccRCC and tRCC cell lines. Data are shown as mean -+ s.d. for n = 4 biological replicates. b, NADPH or NADP+ levels in ccRCC and tRCC cell lines. Data are shown mean -+ s.d. for n = 4 biological replicates. c, Baseline ratio of NADH/NAD+ and NADPH/NADP+ in 5 ccRCC and 3 tRCC cell lines, Data are shown as mean, biological replicates information as for Extended Data Fig. 4a and 4b. d, Levels of NADH and NAD+, as well as the ratio of NADH/NAD+ upon ASPSCR1-TFE3 knockout in FU-UR-1 tRCC cells. Data are shown mean -+ s.d. for n = 4 biological replicates. e, Quantification of NADH to NAD+ ratio following TFE3 knockout in tRCC cell lines (UOK109 and s-TFE). f, Levels of NADH and NAD+, as well as the ratio of NADH/NAD+ upon TFE3 knockout in 786-O ccRCC cells. Data are shown mean -+ s.d. for n = 4 biological replicates. g, Levels of NADPH and NADP+, as well as the ratio of NADPH/NADP+ upon ASPSCR1-TFE3 knockout in FU-UR-1 tRCC cells. Data are shown mean -+ s.d. for n = 4 biological replicates. h, Levels of NADPH and NADP+, as well as the ratio of NADPH/NADP+ upon TFE3 knockout in 786-O ccRCC cells. Data are shown mean -+ s.d. for n = 4 biological replicates. For (c-h), statistical significance was determined by two-sided Mann–Whitney test.
