Extended Data Fig. 4: Controls for organoid stains.
From: Protein-responsive gut hormone tachykinin directs food choice and impacts lifespan
a, bright-field (left) and confocal (right) images of a single entire mouse-intestinal organoid. Serotonin/Substance-P-expressing enterochromaffin cells are marked with CFP expressed under the Tph1 promoter (cyan); rich actin staining with phalloidin to stain F-actin marks the luminal surface of the organoid (orange), and nuclei are marked with DAPI (blue). Scale bars span 50 µm. Similar results were observed in five independent organoid samples. b, Antibody negative control for SP staining. Top row: tissue stained with anti-SP primary (left, orange), also visualized with Tph1-CFP and DAPI for context; bottom row: tissues processed similarly except for the omission of anti-SP. Scale bar indicates 20 µm. Similar results were observed in five independent organoid samples.
