Extended Data Fig. 6: Dietary effects on sleep are mediated by signaling via gut-derived Tk and AKH signaling in males and mated female flies.
From: Protein-responsive gut hormone tachykinin directs food choice and impacts lifespan
Sleep profiles and summed minutes of sleep during the mid-day ‘siesta’ (ZT 4-8) are shown, along with total nighttime (ZT 12-24) sleep. Animals had access to sugar-only food on day 1 and yeast-only food on day 2. a, Nighttime sleep in female controls and flies expressing RNAi against Tk in the Tk+ EECs (two-tailed Mann-Whitney U tests). b, Sleep profile (left) and sleep during the midday ‘siesta’ for female controls and animals expressing RNAi against Tk in the Tk+ EECs (two-tailed Mann-Whitney U tests). c-e, Sleep profile (c) midday sleep (d), and nighttime sleep (e) for males expressing RNAi targeting Tk in the midgut EECs (Kruskal-Wallis/Dunn’s). f, Nighttime sleep for female controls and animals expressing RNAi against TkR99D in the APCs (two-tailed Mann-Whitney U tests). g-i, Sleep profile (g), midday sleep (h), and nighttime sleep (i) for female controls and animals expressing RNAi against TkR99D in the APCs (two-tailed Mann-Whitney U tests). j, Nighttime sleep in female controls and animals expressing RNAi against Akh in the APCs (two-tailed Mann-Whitney U tests). k, Nighttime sleep for female controls and animals expressing RNAi against AkhR in the fat body (two-tailed Mann-Whitney U tests). l-n, Sleep profile (l), midday sleep (m), and nighttime sleep (n) for female controls and animals expressing RNAi against AkhR in the fat body (two-tailed Mann-Whitney U tests). o, Nighttime sleep for female controls and animals expressing RNAi against AkhR in all neurons (two-tailed Mann-Whitney U tests). p-r, Sleep profile (p), midday sleep (q), and nighttime sleep (r) for female controls and animals expressing RNAi against AkhR in all neurons (two-tailed Mann-Whitney U tests). Shaded areas in sleep profiles indicate SEM. Sample sizes (N) and p values are indicated in each plot. In all panels, N = animals per group. Data in dot plots are presented with medians and 95%-confidence intervals. Two-way ANOVA was used to determine interaction between genotype and diet. ns, non-significant (p > 0.05).
