Extended Data Fig. 7: AKH signaling in cholinergic and octopaminergic neurons does not mediate dietary effects on sleep in mated female flies.
From: Protein-responsive gut hormone tachykinin directs food choice and impacts lifespan
Sleep profiles and summed minutes of sleep during the mid-day ‘siesta’ (ZT 4-8) and total nighttime sleep are shown. During the first day of the assay, animals had access to sugar-only medium within the sleep-monitoring apparatus, and on the second day, the animals had access to yeast-only food. a-c, Sleep profile (a), midday sleep (b), and nighttime sleep (c) for female controls and animals expressing RNAi targeting AkhR in the cholinergic fraction of the nervous system using ChAT > (Kruskal-Wallis/Dunn’s). d-f, Sleep profile (d), midday sleep (e), and nighttime sleep (f) for female controls and animals expressing RNAi against AkhR in the octopaminergic neuronal population using Tdc2 > (Kruskal-Wallis/Dunn’s). Shaded areas in sleep profiles indicate SEM. Sample sizes (N) and p values are indicated in each plot. In all panels, N = animals per group. Data in dot plots are presented with medians and 95%-confidence intervals. Two-way ANOVA was used to determine interaction between genotype and diet. ns, non-significant (p > 0.05).
