Extended Data Fig. 8: Offspring number and lifespan in mated females flies with pan-neuronal TkR99D knockdown and significance p values related to Fig. 8a-c.

a-c, p values (a) p values for Fig. 8a, (b) p values for Fig. 8b, and (c) p values for Fig. 8c (one-way ANOVA/Dunnett’s). d, Analysis of fecundity in mated females expressing pan-neuronal TkR99D knockdown under the control of R57C10 > [two-way ANOVA (genotype × days) with Dunnett’s multiple-comparisons test]. e, Lifespan of animals with and without EEC-specific Tk knockdown, on media containing 10% or 1% yeast. Pairwise p-values derived by log-rank Mantel-Cox tests. f, Quantification of calcium-dependent activity (Tk>CaLexA), as indicated by the GFP:tdTomato ratio, in Tk⁺ EECs of R5, along with the intensity of anti-Tk staining, in flies fed on diets containing 10% sugar and either 10% or 20% yeast for 15 h (two-tailed Mann-Whitney U tests). g, Quantification of calcium-dependent activity, as indicated by the GFP:tdTomato ratio (driven by NPF-GAL4), in the middle midgut (R3) of flies assayed as in panel f (two-tailed Mann-Whitney U). h-j, Lifespan of female flies kept on medium containing either 10% or 1% yeast (with 10% sugar in both cases). h, controls and animals expressing RNAi targeting TkR99D in the APCs; i, flies expressing RNAi against AkhR pan-neuronally with R57C10> vs. controls; and j, animals with or without knockdown of AkhR in the fat body. Pairwise p-values in h-j determined by log-rank Mantel-Cox survival tests. Sample sizes (N) and p values are indicated. In d, N = independent groups of animals. In e, i, and j, N = animals per group. In f, N = EECs. Panel d indicates means ± SEM, whereas panels f and g indicate median and 95%-confidence intervals. ns, non-significant (p > 0.05).

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