Extended Data Fig. 5: Gipr CNS KO mice are resistant to mGIPR antibody treatment.
Male Giprfl/fl and GiprSyn−/− littermates were fed HFD for 13 weeks and then treated with vehicle (2 mL/kg) or mGIPR-Ab (25 mg/kg) every 6 days for 48 days. (a) Day 48 % change in body weight, (b) body weight over time, and (c) average daily food intake measured every 3 days. Body composition measured by MRI (d) fat mass and (e) lean mass on days -3 and 46. Day 48 necropsy tissue weights (f) epididymal WAT, (g) inguinal WAT, and (h) liver. Day 48 4-hour fasted (i) blood glucose, (j) plasma insulin, and (k) plasma total cholesterol. (A-K) Giprfl/fl / Vehicle (n=8), Giprfl/fl / mGIPR-Ab (n=7), GiprSyn−/− / Vehicle (n=14), GiprSyn−/− / mGIPR-Ab (n=14). (A, D-K) One-way ANOVA with Tukey’s test for multiple comparisons, (B-C) Two-way repeated measures ANOVA or mixed-effects analysis with Tukey’s test for multiple comparisons. *p <0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. vehicle within genotype or comparison indicated by bracket; + (black) vs. Giprfl/fl within vehicle; + (red) vs. Giprfl/fl within mGIPR-Ab treatment. Data represent mean ± SEM.
