Fig. 2: Mice with CNS KO of Gipr are resistant to diet-induced obesity. | Nature Metabolism

Fig. 2: Mice with CNS KO of Gipr are resistant to diet-induced obesity.

From: GIPR-Ab/GLP-1 peptide–antibody conjugate requires brain GIPR and GLP-1R for additive weight loss in obese mice

Fig. 2: Mice with CNS KO of Gipr are resistant to diet-induced obesity.

a, Breeding schematic for generation of CNS Gipr KO mice (created with BioRender.com). Male Giprfl/fl and GiprSyn−/− littermates were fed HFD for 12 weeks. b,c, Gipr RNA expression from isolated pancreatic islets (b) and brainstem, hypothalamus and hippocampus (c) were assessed to confirm CNS Gipr KO. d, Body weight, e, food intake, f, fat mass and g, lean mass were measured throughout HFD feeding. For b, n = 6 mice per genotype; for c, Giprfl/fl (n = 5 per brain region), GiprSyn−/− (n = 6 brainstem, n = 7 hypothalamus and hippocampus); for d–g, Giprfl/fl (n = 11) and GiprSyn−/− (n = 10). In b, multiple unpaired t-tests with a 1% false discovery rate for multiple comparisons; in c–f, two-way ANOVA with Šidák’s test for multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data represent mean; error bars, s.e.m.

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