Extended Data Fig. 2: mGIPR-Ab/P1 and mGIPR-Ab/P3 have similar in vitro potency and comparable effects on cAMP activity. | Nature Metabolism

Extended Data Fig. 2: mGIPR-Ab/P1 and mGIPR-Ab/P3 have similar in vitro potency and comparable effects on cAMP activity.

From: GIPR-Ab/GLP-1 peptide–antibody conjugate requires brain GIPR and GLP-1R for additive weight loss in obese mice

Extended Data Fig. 2: mGIPR-Ab/P1 and mGIPR-Ab/P3 have similar in vitro potency and comparable effects on cAMP activity.

Potency and relative activity of mGIPR-Ab/P1 and mGIPR-Ab/P3 were compared in vitro. (a) GIPR IC50 and GLP-1R EC50 are comparable with both molecules, despite different GLP-1 analogs used. (b) mGIPR-Ab/P1 and mGIPR-Ab/P3 have identical curves in a GIPR cAMP antagonist assay in 293 T hGIPR-expressing cells, as well as in (c) GLP-1R cAMP agonist assay in CHOK1 hGLP-1R-expressing cells. Assay was run in technical duplicates. cAMP = cyclic AMP; CHO = Chinese hamster ovary.

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