Extended Data Fig. 5: Fiber Photometry measurement of PVNGLP-1R→DVC neuronal calcium activity in responding to various sensory inputs.
From: State-dependent central synaptic regulation by GLP-1 is essential for energy homeostasis
a. Average response of fiber photometry data showing calcium dynamics of PVNGLP-1R→DVC neurons during self-grooming behavior. b. Pooled data (two-tailed paired t-test, t(5) = 2.822, p = 0.0477; n = 5 mice). c. Average response of fiber photometry calcium dynamics of PVNGLP-1R→DVC neurons during tail picking-induced stress d. Pooled data (two-tailed paired t-test, t(5) = 4.109, p = 0.0147; n = 5 mice). e. Heatmap showing calcium dynamics of PVNGLP-1R→DVC neurons during different food/object tea ball drop stimuli across different energy states. f. Quantitation of the areas under the curve (AUC) of calcium response (one-way ANOVA, F(3,16) = 4.383, p = 0.0197, n = 5 mice). Data are presented as mean ± SEM. ∗p < 0.05.
