Fig. 1: SANA protects against DIO.
a, Mice fed HFD, HFD + salicylate (SAL) or HFD + SANA (400 mg per kg per day, mixed in HFD, p.o.) for 8 weeks. b, Weight gain. n: HFD = 30; HFD + SAL = 29; HFD + SANA = 30; pooled from three experiments. c, X-ray image (left) and tibia length (right). n = 9 per group. d,e, Weight gain under normal diet (ND), HFD or HFD + SANA (d) or HFD + SAL (e) at different doses administered p.o. n: HFD = 8; HFD + SANA 200 mg per kg per day = 6; HFD + SANA 300 mg per kg per day = 8; HFD + SANA 400 mg per kg per day = 10; ND = 8 in d, and HFD = 11; HFD + SAL 200 mg per kg per day = 5; HFD + SAL 300 mg per kg per day = 9; HFD + SAL 400 mg per kg per day = 10; ND = 8 in e. f, Cumulative food intake. n = 4 per group. g, Stool production (left) and caloric content (right) at week 8. n: HFD = 9; HFD + SAL = 11; HFD + SANA = 11 and HFD = 7; HFD + SAL = 6; HFD + SANA = 6, respectively. h, Digested energy calculated as the percentage of gross energy intake at week 8. n = 6 per group. i, Representative images; arrows indicate perigonadal fat. j, Total fat mass by EchoMRI. n = 8 per group. k, Fat depot quantification: interscapular brown (iScap_BAT), inguinal (i_WAT), subscapular (sScap_WAT) and perigonadal (p_WAT) adipose tissue. n: iScap_BAT: HFD = 10; HFD + SAL = 14; HFD + SANA = 11; i_WAT: HFD = 10; HFD + SAL = 14; HFD + SANA = 12; sScap_WAT: HFD = 10; HFD + SAL = 14; HFD + SANA = 12 and p_WAT: HFD = 10; HFD + SAL = 13; HFD + SANA = 10. l, Total lean mass by EchoMRI. n = 8 per group. m, Mice fed ND or ND + SANA (400 mg per kg per day, p.o.) for 8 weeks. n, Weight at start and end of treatment n: ND = 7; ND + SANA = 8. Data are the mean ± s.e.m. Statistical tests used were two-way analysis of variance (ANOVA) followed by Tukey’s multiple-comparisons test (b, d, e, f and n), repeated-measures one-way ANOVA (c) and one-way ANOVA (g, h, j, k and l). One-way ANOVA was followed by Bonferroni post hoc for multiple comparisons.
