Fig. 4: Dietary fat modulates acylcarnitine levels in plasma of DIO mice.
From: The source of dietary fat influences anti-tumour immunity in obese mice
a, Long-chain and very long-chain fatty acid composition of HFDs derived from butter and palm oil. b–d, Plasma metabolome of mice fed HFD derived from butter, palm oil or SFD for 1 week, analysed by mass spectrometry: PCA plot of plasma metabolomes of mice fed butter-HFD and palm oil-HFD (b); volcano plot of differentially expressed metabolites in mice fed butter-HFD (pink) and palm oil-HFD (blue); and relative abundance of CAR18:0 (d). e, Relative abundance of CAR18:0 in serum of B16-F10 tumour-bearing mice fed butter, palm oil or SFD for 12 weeks. f–h, Plasma metabolome of mice fed HFD based on lard (60% fat) or SFD for 8 weeks, analysed by mass spectrometry: PCA plot of plasma metabolomes of lard-fed (orange) and SFD-fed (grey) mice (f); volcano plot of metabolites differentially expressed in lard-fed (orange) and SFD-fed (grey) mice (g); and relative abundance of CAR18:0 in lard and SFD (h). Sample sizes are as follows: in a, n = 4; in b–d, SFD n = 5, butter n = 5, palm oil n = 4; in e, SFD n = 4, butter n = 5, palm oil n = 5; in f–h, n = 10. In bar graphs, bar height represents the mean; error bars, s.e.m. Comparisons were made using one-way ANOVA followed by Tukey’s multiple comparisons test or the Mann–Whitney test.
