Extended Data Fig. 2: High fat diet causes obesity and impairs systemic metabolism, regardless of dietary fat source.
From: The source of dietary fat influences anti-tumour immunity in obese mice
a-h, Male mice were fed HFD derived from Butter, Palm Oil, or SFD for 12 weeks. Weight of visceral (a) and subcutaneous (b) adipose tissue at study endpoint. (c) Average daily food consumption measured 6 weeks after diet initiation. (d) Bomb calorimetry of faeces collected after 10 weeks on diet. (e) Area under the curve of glucose tolerance test, performed 10 weeks after diet initiation. Fasting serum insulin (f) and insulin tolerance test (g) performed 10 weeks after diet initiation. (h) Representative oil red O staining (left) and summary data (right) of livers from HFD-fed mice. i, j, Mice were fed assigned diet for one week and singly housed for indirect calorimetry. Hourly (i, left) and average daily (i, right) locomotor activity. Hourly (j, left) and average daily (j, right) energy expenditure. (a, b) n = 10; (c) SFD n = 4, Butter n = 7, Palm Oil n = 7, data pooled from 2 independent experiments; (d) n = 7, (e) n = 5; (f) n = 4-5; (g, left) n = 10, combined from 2 independent experiments; (g, right) n = 5, representative of 2 independent experiments; (h) SFD n = 3, Butter n = 5, Palm Oil n = 5; (i-j) n = 4, representative of 2 independent experiments. In bar graphs, bar height represents mean with error bars ± SEM. In line graphs, each data point represents the mean with error bars ± SEM. Comparisons were made using one-way ANOVA followed by Tukey’s multiple comparisons test (a-j) or two-way repeated measures ANOVA followed by Bonferroni’s multiple comparisons test (g, left).
