Fig. 1: Dynamic itaconate metabolism and correlation with succinate levels in infused rat models.
From: In vivo itaconate tracing reveals degradation pathway and turnover kinetics
a, Plasma metabolite abundances at 30 min compared to 0 min after itaconate infusion. b, Plasma itaconate levels over time with two 30 min itaconate infusions (indicated in grey). c, Plasma succinate levels over time with two itaconate infusions. d, Relative level of methylmalonate in plasma over time with two itaconate infusions. e, Plasma level of leucine, isoleucine and valine over time with two itaconate infusions. f, Relative mesaconate level over time with two itaconate infusions. g, Schematic depicting the impact of itaconate on SDH and MUT activities. Experiments were performed with n = 4 rats; itaconate infusion (15 mg kg−1 min−1 for 30 min) was performed twice (indicated in grey). Data are presented as boxplots (25th to 75th percentile with median line) and whiskers (min. to max. values) (a) or mean ± s.e.m. (b–f) obtained from n = 4 rats. P values were calculated by multiple paired t-tests (a) or two-way ANOVA compared to 0 min with Fisher’s least significant difference (LSD) post hoc test (b–f); *P < 0.05; **P < 0.01; ***P < 0.001. Exact P values are indicated in each figure panel or in Extended Data Fig. 1f–h (e).
