Fig. 1: Lipotypes of human iNeurons, iAstrocytes and microglia.
From: The Neurolipid Atlas: a lipidomics resource for neurodegenerative diseases
a, Schematic overview of the Neurolipid Atlas workflow and resource. b, Schematic overview of iPS cell differentiation protocols. c, Representative confocal microscopy images of iNeurons, iAstrocytes and iMicroglia in monoculture of at least three independent differentiations per cell type. Scale bar, 50 μm (BIONi037-A parental line). d, Heat map of z-scored lipid class abundance in iNeurons, iAstrocytes and iMicroglia (BIONi037-A parental line). e, PCA analysis of iPS cell-derived brain cell lipotypes. f, Pie charts showing relative abundance of all detected lipid classes in the iPS cell-derived brain cell types. g, Bar graphs present individual lipid class levels in each cell type, normalized to total lipid level. N (iNeurons), n = 4 wells; A (iAstrocytes), n = 3 wells; M (iMicroglia), n = 4 wells. Data are shown as the mean ± s.d. Images in a,b were created using BioRender.com.
