Extended Data Fig. 3: Extended analysis of human isogenic APOE3/3 and APOE4/4 iAstrocytes.

a) Representative sequencing result for confirming cell identity and expected ApoE genotype. b) Representative images of differentiated iAstrocytes, from both Kolf2.1J (set #1) and BIONi037 lines. Scale bar = 50μm. c) Gene expression levels (as determined by RNAseq) of indicated mature and immature astrocyte markers in our iPSC-derived astrocytes. n=6 wells BIONi037 (n=3 ApoE3, n=3 ApoE4). d) Relative ApoE levels secreted in the medium. N=3, one or two independent experiments from two isogenic sets. Mean e) Percentage of LD positive (>5 LDs quantified) iAstrocytes. N=6, three independent experiments from two isogenic sets. Mean **P<0.01 t-test. Data corresponds to Fig. 3h. In all panels open triangles indicate mean per experiment, while solid dots represent all independent wells.