Extended Data Fig. 6: β-OHB contributes to PUFA elongation through both OXCT1 and AACS. | Nature Metabolism

Extended Data Fig. 6: β-OHB contributes to PUFA elongation through both OXCT1 and AACS.

From: An alternative route for β-hydroxybutyrate metabolism supports cytosolic acetyl-CoA synthesis in cancer cells

Extended Data Fig. 6

Related to Fig. 3. a-d, [M + 2] fractional labeling of 20:3(n-6), 20:4(n-6), 22:4(n-6), and 22:6(n-3) in the indicated AL1376 knockout cells labeled with 5 mM [U-13C]-β-OHB for 48 h in lipid-replete media. e-g, [M + 2] fractional labeling of 20:3(n-6), 20:4(n-6), 22:4(n-6), and 22:6(n-3) in the indicated B16 knockout cells labeled with 5 mM [U-13C]-β-OHB for 48 h in lipid-replete media. Data are presented as mean ± s.e.m; n = 3 biologically independent replicates. Comparisons were made using a two-tailed Student’s t test (a, d, g) or a one-way ANOVA (b, c, e, f).

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