Extended Data Fig. 7: β-OHB can contribute to cytosolic acetyl-CoA production through a citrate-independent route that requires AACS.
Related to Fig. 3. a, Immunoblot for BDH1 and vinculin in AL1376 sgBdh1 #1 cells with or without BDH1-HA re-expression. b, c, Citrate mass isotopomer distribution (MID) (b) and cytosolic acetyl-CoA MID (c) in AL1376 sgBdh1 #1 cells, with or without BDH1-HA re-expression, labeled with 5 mM [U-13C]-β-OHB for 48 h in lipid-replete or lipid-depleted culture media. d, Immunoblot for OXCT1 and vinculin in AL1376 sgOxct1 #1 cells with or without OXCT1-HA re-expression. e, f, Citrate MID (e) and cytosolic acetyl-CoA MID (f) in AL1376 sgOxct1 #1 cells, with or without OXCT1-HA re-expression, labeled with 5 mM [U-13C]-β-OHB for 48 h in lipid-replete or lipid-depleted culture media. g, Immunoblot for AACS and vinculin in AL1376 sgAacs #1 cells with or without AACS-HA re-expression. h, i, Citrate MID (h) and cytosolic acetyl-CoA MID (i) in AL1376 sgAacs #1 cells, with or without AACS-HA re-expression, labeled with 5 mM [U-13C]-β-OHB for 48 h in lipid-replete or lipid-depleted culture media. Data are presented as mean ± s.e.m; n = 3 biologically independent replicates. Comparisons were made using a two-tailed Student’s t test (b, c, e, f, h, i).
