Extended Data Fig. 9: β-OHB is available in the plasma and tumor interstitial fluid in tumor-bearing mice.
Related to Fig. 4. a, β-OHB concentrations in tumor interstitial fluid (TIF) collected from subcutaneous or orthotopic AL1376 tumors growing in C57BL/6 J mice. Subcutaneous TIF n = 6, Orthotopic TIF n = 2. b, Total fatty acid concentrations (free + saponified) in tumor interstitial fluid (TIF) collected from subcutaneous or orthotopic AL1376 tumors growing in mice. Subcutaneous TIF n = 5, Orthotopic TIF n = 2. c, d, Body weights of mice bearing the indicated subcutaneous B16 tumors exposed to a control or caloric restriction (CR) diet. (c) sgNTC Control n = 6 mice, CR n = 6 mice; sgBdh1 #1 Control n = 6 mice, CR n = 6 mice; sgOxct1 #1 Control n = 5 mice, CR n = 5 mice; sgAacs #1 Control n = 6 mice, CR n = 6 mice. (d) sgNTC Control n = 3 mice, CR n = 4 mice; sgOxct1/Aacs Control n = 4 mice, CR n = 4 mice. e-g, Concentrations of glucose (e), β-OHB (f), and total fatty acids (free + saponified) (g) in the plasma from mice fed a control or CR diet. Control n = 6, CR n = 5. h, Immunoblot for BDH1, OXCT1, AACS, and vinculin in B16 sgNTC tumors from mice fed a control or CR diet. Data are presented as mean ± s.e.m (b-d, g) or as box-and-whisker plots displaying median, interquartile range (boxes), and minima and maxima (whiskers) (a, e, f). Comparisons were made using a two-way ANOVA (c, d) or a two-tailed Student’s t test (e-g).
