Fig. 2: β-OHB is a major source of cytosolic acetyl-CoA.
a–c, Palmitate (16:0) MID for B16 (a), AL1376 (b) and MIA PaCa-2 (c) cells labelled with 5 mM [U-13C]-β-OHB for 48 h in lipid-replete versus lipid-depleted culture media. d–f, Citrate MID (solid bars) and cytosolic acetyl-CoA MID (dashed bars) for B16 (d), AL1376 (e) and MIA PaCa-2 (f) cells labelled with 5 mM [U-13C]-β-OHB for 48 h in lipid-replete versus lipid-depleted culture media. g–j, 16:0 MID (g,i) and citrate and cytosolic acetyl-CoA MID (h,j) for B16 cells labelled with 10 mM [U-13C]-glucose for 48 h in lipid-replete versus lipid-depleted culture media, either without unlabelled β-OHB (g,h) or with 5 mM unlabelled β-OHB (i,j). k, Cytosolic acetyl-CoA label dilution from citrate, as calculated by the log2(fold change) of the total fraction of cytosolic acetyl-CoA labelled versus the total fraction of citrate labelled, in B16, AL1376 and MIA PaCa-2 cells under the indicated tracing conditions. Data are presented as means; error bars, s.e.m.; n = 3 biologically independent replicates. Comparisons were made using a two-tailed Student’s t-test (d,e,f,h,j) or a two-way ANOVA (k).
