Extended Data Fig. 5: β-OHB can contribute to cytosolic acetyl-CoA production in B16 cells through a citrate-independent route that requires AACS in lipid-replete media. | Nature Metabolism

Extended Data Fig. 5: β-OHB can contribute to cytosolic acetyl-CoA production in B16 cells through a citrate-independent route that requires AACS in lipid-replete media.

From: An alternative route for β-hydroxybutyrate metabolism supports cytosolic acetyl-CoA synthesis in cancer cells

Extended Data Fig. 5

Related to Fig. 3. a-c, Citrate mass isotopomer distribution (MID) (a), palmitate (16:0) MID (b), and cytosolic acetyl-CoA MID (c) in B16 sgNTC, sgBdh1 #1, and sgBdh1 #2 cells labeled with 5 mM [U-13C]-β-OHB for 48 h in lipid-replete culture media. d-f, Citrate MID (d), 16:0 MID (e), and cytosolic acetyl-CoA MID (f) in B16 sgNTC, sgOxct1 #1, and sgOxct1 #2 cells labeled with 5 mM [U-13C]-β-OHB for 48 h in lipid-replete culture media. g-i, Citrate MID (g), 16:0 MID (h), and cytosolic acetyl-CoA MID (i) in B16 sgNTC, sgAacs #1, and sgAacs #2 cells labeled with 5 mM [U-13C]-β-OHB for 48 h in lipid-replete culture media. j-l, Citrate MID (j), 16:0 MID (k), and cytosolic acetyl-CoA MID (l) in B16 sgNTC and sgOxct1/Aacs cells labeled with 5 mM [U-13C]-β-OHB for 48 h in lipid-replete culture media. Data are presented as mean ± s.e.m; n = 3 biologically independent replicates. Comparisons were made using a two-tailed Student’s t test (a, c, d, f, g, i, j, l).

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