Extended Data Fig. 2: mRNA and protein expression of ZZEF1 in human islets.
From: Single-cell perturbations decipher ribosomal stress-surveillance regulators in type 2 diabetes
a, Normalized expression levels of ZZEF1 (FPKM from RNA-seq or log2 RMA signal intensity from oligo microarray) in human islets from non-diabetic (ND), impaired glucose tolerance (IGT) and T2D individuals. PPP, partially pancreatectomized patient. OD, organ donor. RMA, robust multi-array average. P values were calculated by two-sided Wilcoxon test (GSE50244 n = 52, 15, 10; GSE159984 n = 58, 27; GSE164416 n = 18, 38, 39) or the empirical Bayes moderated two-sided t-test in limma package (GSE76896 PPP group n = 32, 15, 36; OD group n = 84, 19). b, Relative protein levels of ZZEF1 (log2 normalized abundance from bulk proteomics) in human islets from male and female ND and T2D individuals (n = 77, 40, 10, 7). P values were calculated using the empirical Bayes moderated two-sided t-test in limma package. Boxplots display the median (center line), interquartile range (IQR, box edges), whiskers extending to the most extreme values within 1.5 × IQR. Data points past the whiskers are considered outliers and are plotted individually. c,d, Representative immunohistochemical staining of ZZEF1 on the pancreatic sections from male and female ND and T2D individuals. Scale bars, 50 μm (c). H-scores were analyzed in islets of different sections (n = 8, 8, 7, 9) (d). e-h, Representative IF staining of ZZEF1 (green), C-pepteide (red) and DAPI (blue) on pancreatic sections from male and female ND and T2D individuals. Scale bars, 50 μm (e). The mean fluorescence intensity of ZZEF1 was analyzed (n = 8, 8, 5, 6) (f). The proportions of ZZEF1+ cells in islets (g) and ZZEF1+C-peptide+ cells in C-peptide+ cells (h) were analyzed (n = 8, 8, 5, 6). The number of samples per group is indicated at the bottom of bars in the graphs. Data represented as mean ± S.D. P values were calculated by unpaired two-tailed Student’s t-test (2 groups).
