Extended Data Fig. 6: scRNA-seq analysis of islet cells in βZKO-MIP and βZKO-RIP mice.
From: Single-cell perturbations decipher ribosomal stress-surveillance regulators in type 2 diabetes
a, The UMAP of islet cells from βZKO-MIP and control mice fed with NCD or HFD (left, 46,393 cells), βZKO-RIP and control mice fed with NCD or HFD (right, 44,242 cells). Each group consists of two replicates; each replicate contains islets from at least three mice. b, Proportion of cell types in the islets from βZKO-MIP, βZKO-RIP and control mice fed with NCD or HFD. Alpha, α-cells; Beta, β-cells; Delta, δ-cells; PP, pancreatic polypeptide cells. c,d, Bubble plot of marker genes expression in the islet cell types of βZKO-MIP (c), βZKO-RIP (d) and control mice. e,f, Proportions of α, β, δ, PP cells and insulin+glucagon+ (INS+GCG+) in the flow cytometry analysis of islets from HFD-fed βZKO-MIP (e), βZKO-RIP (f) and control mice (n = 3 for each group). P values were calculated by unpaired two-tailed Student’s t-test. g, The separated UMAP of insulin-expressing cells based on scRNA-seq of islets from NCD-fed 12-week-old βZKO-MIP, βZKO-RIP and control mice, and HFD-fed 20-week-old βZKO-MIP, βZKO-RIP and control mice. h, Proportion of 4 β-cell subtypes in the islets from βZKO-MIP, βZKO-RIP and control mice fed with NCD or HFD. i, Bubble plot of representative marker genes for the β-cell subtypes of βZKO-MIP, βZKO-RIP and control mice fed with NCD or HFD. j, Enriched GO terms of common DEGs between Zzef1-KO and control β-cells from βZKO-MIP, βZKO-RIP mice fed with NCD or HFD. P values were calculated by one-sided hypergeometric test. k, Violin plots of Ins1 and Ins2 expression in β-cells of βZKO-MIP, βZKO-RIP and control mice fed with NCD or HFD (βZKO-MIP n = 7,160, 9,436, 9,855, 7,370; βZKO-RIP n = 6,495, 5,578, 7,420, 11,942). P values were calculated by two-sided Wilcoxon rank-sum test. Boxplots display the median (center line), IQR (box edges), whiskers extending to the most extreme values within 1.5 × IQR. Data points beyond the whiskers are considered outliers and are plotted individually. All mice were male. The number of samples per group is indicated at the bottom of bars in the graphs. Data represented as mean ± S.D.
