Extended Data Fig. 8: Azoramide treatment restores β-cell function in mice.
From: Single-cell perturbations decipher ribosomal stress-surveillance regulators in type 2 diabetes
a, Schematic schedule for azoramide treatments and experiments in mice. b,c, Body weights of the βZKO-MIP and control mice treated with azoramide or vehicle (n = 5, 6, 7, 6) (b), or ISRIB or vehicle (n = 5, 6, 6, 6) (c). d, Representative RNAscope FISH of hormone genes (Gcg, Ins2, Ppy, Sst) on the pancreatic sections of βZKO-MIP and littermates control mice treated with azoramide or vehicle. Scale bars, 20 μm. e,f, IF staining of insulin (white), cocktail of GCG/SST/PPY (red), CHGA (green) and DAPI (blue) on pancreatic sections of HFD-fed βZKO-MIP and control mice (e). The yellow arrows point at the CHGA+ hormone− dedifferentiated cells, and their proportion in islets was analyzed (n = 3 for each group) (f). Scale bars, 20 μm. All mice were male. The number of samples per group is indicated at the bottom of bars in the graphs. Data represented as mean ± S.D. P values were calculated by unpaired two-tailed Student’s t-test.
