Extended Data Fig. 1: Quality control and analysis for Perturb-seq screening of T2D susceptibility genes.
From: Single-cell perturbations decipher ribosomal stress-surveillance regulators in type 2 diabetes
a, Cell proportion of detected sgRNA for each targeting gene. b, Cell proportion of 7 replicate Perturb-seq data for each targeting gene. c, Cell proportion of 7 EndoC-βH1 clusters for each targeting gene. d, The number of perturbed cells for each targeting gene and non-targeting control. The gray dashed line represents a filtering threshold of 50 cells. e, The enriched GO terms of perturbation effects of CALCOCO2, SLC30A8, SND1 and THADA in EndoC-βH1 cells. P values were calculated by one-sided hypergeometric test. BP, biological process.
