Fig. 4: SLC25A51 overexpression promotes fatty acid metabolism and attenuates the depletion of mitochondrial metabolites during liver regeneration.
From: Hepatocyte mitochondrial NAD+ content is limiting for liver regeneration
The changes in metabolites and protein expression in livers and isolated hepatic mitochondria at 48 h post PHx in mice infected with control AAV (eGFP) or overexpressing SLC25A51 in hepatocytes (OE) are shown. a,b, The metabolite changes during regeneration in control (a) and SLC25A51-overexpressing (b) liver tissue. c, The correlation plots of log2 fold changes in liver. d,e, The protein changes during regeneration in control (d) and SLC25A51-overexpressing (e) liver tissue. f, The principal component analysis (PCA) of liver proteomes showing principal components 1 and 2 (PC1, PC2). GFP.R and OE.R indicate the regenerated livers. g,h, The expression of proteins involved in fatty acid biosynthesis in SLC25A51-overexpressing resected (g) and regenerated (h) livers. i,j, The metabolite changes in mitochondria during regeneration of control (i) and SLC25A51-overexpressing (j) livers. k, The correlation plots of log2 fold changes in the metabolites of isolated liver mitochondria. The volcano plots show metabolites (in closed circles) and proteins (in open circles) that were significantly changed with a P value less than 0.05 coloured in red (upregulated) or in green (downregulated). The correlation plots of log2 fold change in OE versus eGFP were made by selecting all significant metabolites within the eGFP comparison alone, OE comparison alone or shared (common) for both liver tissue and isolated mitochondria. The metabolites and proteins that were significantly changed with a P value less than 0.05 were determined by two-tailed Student t-tests. The individual P values are shown where significant (P < 0.05) (n = 3–5 mice per group for eGFP control and A51 OE, n = 6–8 mice per group).
