Extended Data Fig. 1: SLC25A47 knockdown or overexpression has no impact on mitochondrial NAD+ levels.
From: Hepatocyte mitochondrial NAD+ content is limiting for liver regeneration
a-b, Blood glucose (a) and lactate (b) measurements (n = 10 for A51+/+ and n = 9 for A51+/-). c-d, Whole cell (c) and mitochondrial (d) NAD+ measured in HeLa cells using a luminescence assay (n = 6 for all groups). e, mRNA validation of siRNA knockdown of Slc25a51 (n = 3 for all groups). f, Western blot showing enrichment of Cox IV (mitochondrial) and depletion of GAPDH (cytosolic) in mitochondrial fractions isolated from HeLa cells. g-h, mRNA validation of siRNA knockdown of Slc25a47 (g) and Slc25a51 (h) in HepG2 cells (n = 3 for all groups). i, Western blot showing overexpression of SLC25A51 and SLC25A47 in HepG2 cells. Data is presented as the mean and error bars represent s.e.m. Individual P values are shown where significant (P ≤ 0.05). Tests for normality and equality of variances, along with the resulting statistical test selected for each panel are provided in statistical source data.
