Extended Data Fig. 3: Nucleotide salvage is active in AML cells.
a) Effect of Complex II inhibition by TTFA on the steady state level of de novo purine biosynthesis product IMP. b-c) Effect of exogenous purines (adenine (A), guanine (G), hypoxanthine (H), inosine (I)) or pyrimidines (cytidine (C), uridine (U), thymidine (T)) on viability in (b) OCI-AML2 cells or (c) MOLM-13 cells treated with the targeted purine synthesis (GART) inhibitor, lometrexol. Each nucleotide species was added back at a concentration of 30 μM. d) Effect of exogenous purines or pyrimidines on viability of MOLM-13 cells treated with the pyrimidine synthesis inhibitor, brequinar (2 μM). All data in show mean ± s.e.m. from n = 3 independent experiments. P values were calculated using unpaired, two-tailed Student’s t-test (a) or a one-way ANOVA with a Tukey’s post-hoc test (b-d).
