Fig. 6: Glu interacts widely within the AML proteome.
a, Overview of isothermal proteome profiling method to detect protein–metabolite interactions of Glu or Gln in AML cells. b, Heat map showing the log2FC in abundance after Glu or Gln binding for n = 287 enriched AML proteins (z-score ≥ 2 or ≤ −2 and P value ≤ 0.05 for either Glu or Gln versus control, based on limma analysis). c, Volcano plot showing the top Glu-interacting proteins relative to control. P values were generated using limma. d, STRING-generated protein–protein interaction network for Glu-interacting proteins with z-score ≥ 2 and P value ≤ 0.05. Edges show physical interactions with highest interaction strength (≥0.9) and disconnected nodes are not shown. Two representative pathways, GO:BP ‘cytoplasmic translation’ and GO:CC ‘proteasome complex’, are shown with pathway components highlighted in respective colours and STRING interaction score listed. e, Lollipop plot showing the log2FC and enrichment for the proteins making up the nucleobase metabolic process GO category. P values were generated between control and Glu-treated cells using limma. NES, normalized enrichment score. Panel a created with BioRender.com.
