Extended Data Fig. 2: CD8⁺ T cells mediate the anti-tumor effects of DR.

A) Weighted nearest neighbor Uniform Manifold Approximation and Projection (wnnUMAP) of 45,455 CD45⁺ tumor-infiltrating cells from B16 melanoma tumors of mice on AL or DR diets (wnnUMAP combined; n = 4 mice per diet). Each plot represents the expression of key antibody-derived tags (ADTs) for markers including NK1.1, Ly6C, LAG3 (CD223), CD4, CD8, PD1, F4/80, CD11c, TIM3 (CD366), CD11b, CD69, and CD127. B) Density plots generated using the Nebulosa package, showing the expression density of ADTs (CD4, CD8, CD11c, CD11b, CD127) among activated CD8⁺ T cells from B16 tumors (combined AL and DR conditions). C) Density plots of gene expression for Cd8a, Tbx21, Tox, Prf1, and Il7r among activated CD8⁺ T cells from B16 tumors (joint plot of AL and DR conditions). D) Representative histogram of TBET expression in PD1^LowTOX^Low CD8⁺ TILs from B16 tumors. Bar plots show relative geometric mean fluorescence intensity (gMFI) compared to AL controls. Data represent the mean ± SEM (n = 8 mice per group). E) Representative histogram of IFN-γ expression in PD1^LowTOX^Low CD8⁺ TILs from B16 tumors. Bar plots show relative gMFI compared to AL controls. Data represent the mean ± SEM (n = 8 mice per group). Statistical significance was calculated via two-tailed, unpaired Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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