Extended Data Fig. 3: , related to Fig. 1: Lipid oxidation level.
From: Neuronal fatty acid oxidation fuels memory after intensive learning in Drosophila
Extended Data Fig. 3a-b. Inhibition of glial import of oxidized lipid via GLaz in adult cortex glia using two different RNAi had no effect on memory after massed training (a, n = 7,7,6, F2,17 = 0.69, P = 0.515, b. n = 8, F2,21 = 0.17, P = 0.842,) c. Lipid oxidation state in the soma region of MB neurons measured by C11-BODIPY581/591 (labelling both cell membrane and lipid droplets of cortex glia and neurons) was not affected by associative massed training as compared to unpaired controls (n = 7,8, t13 = 0.99, P = 0.339). scale bar: 15 µm. Data on barplots are expressed as mean ± s.e.m. with dots as individual values, and analyzed by ANOVA with post hoc testing by Newman–Keuls pairwise comparisons test (a-b) or by two-sided unpaired t-test (c). Genotype sample sizes are listed in the legend in order of bar appearance. Significance level of a two-sided unpaired t-test (c) or the Newman–Keuls pairwise comparison between the genotype of interest and the genotypic controls (a-b) following one-way ANOVA: NS: not significant.
