Extended Data Fig. 5: , related to Fig. 3: Control experiments for intracellular FA delivery in MB neurons to sustain memory formed after massed training.
From: Neuronal fatty acid oxidation fuels memory after intensive learning in Drosophila
Extended Data Figure 5a. When Bmm RNAi expression was not induced, memory after massed training was normal (n = 12, F2,33 = 0.67, P = 0.518). b. A second non-overlapping RNAi targeting Bmm (Bmm RNAi GD5139) was used to confirm the specific defect in memory after massed training. As previously carried out in17 using this RNAi line, Dicer2 expression was induced together with RNAi expression in adult MB neurons (Tub-Gal80ts; UAS-Dcr2, VT30559 line) to increase RNAi efficiency. Bmm KD in adult MB neurons impaired memory after massed training (n = 12, F2,33 = 7.21, P = 0.0025), whereas memory after spaced training was normal (n = 14, F2,39 = 1.24, P = 0.300). Non-induced flies showed no memory defect after massed training (n = 9, F2,24 = 1.50, P = 0.244). c. When FABP RNAi expression was not induced, memory after massed training was normal (n = 11, F2,30 = 1.15, P = 0.329). d. A second non-overlapping RNAi targeting FABP (FABP RNAi KK116001) was used to confirm the specific defect in memory after massed training. FABP KD in adult MB neurons impaired memory after massed training (n = 19, F2,54 = 13.51, P = 0.00002), whereas memory after spaced training was normal (n = 16, F2,45 = 1.72, P = 0.190). Non-induced flies showed no memory defect after massed training (n = 13, F2,36 = 2.12, P = 0.135). Data on barplots are expressed as mean ± s.e.m. with dots as individual values, and analyzed by ANOVA with post hoc testing by Newman–Keuls pairwise comparisons test (a-d). Genotype sample sizes are listed in the legend in order of bar appearance. Significance level of the Newman–Keuls pairwise comparison between the genotype of interest and the genotypic controls (a-d) following one-way ANOVA: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 NS: not significant.
