Extended Data Fig. 3: CUMS blunts the survival and functions of liver CD8+ T cells in an extrinsic manner. | Nature Metabolism

Extended Data Fig. 3: CUMS blunts the survival and functions of liver CD8+ T cells in an extrinsic manner.

From: Chronic stress drives liver cancer by impairing the hepatic kynurenine pathway and immune surveillance

Extended Data Fig. 3: CUMS blunts the survival and functions of liver CD8+ T cells in an extrinsic manner.The alternative text for this image may have been generated using AI.

(A-D) Purified liver CD8+T cells from control (labeled with CFSE) or CUMS (labeled with CTV) CD45.2 mice were transferred into CD45.1 recipient mice (n = 6 mice per group). Validation of the purity of mouse liver CD8+T cells for transfer experiment (A). Experimental scheme (B), FACS analysis of CD8+T cell percentage (C) and IFN-γ, TNF-α and GZMB expressions (D) were shown. (E-I) Purified liver CD8+T cells from donor mice (CD45.1) were transferred into control or CUMS mice (CD45.2) (n = 5 mice per group). Experimental scheme (E), the gating strategy of transferred liver CD8+T cell (CD45.1) in liver tissues of recipient mice (CD45.2) (F), FACS analysis of transferred CD8+T cell percentage (G), apoptosis (H) and IFN-γ, TNF, GZMB expressions (I) were shown. Dots represent data from individual mice, and error bars indicate as the mean ± s.e.m. in one experiment. Statistical analyses were done by two-tailed unpaired Student’s t test (C, D, G, H, I).

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