Extended Data Fig. 8: Qprt overexpression promotes hepatic CD8+ T cell homeostasis. | Nature Metabolism

Extended Data Fig. 8: Qprt overexpression promotes hepatic CD8+ T cell homeostasis.

From: Chronic stress drives liver cancer by impairing the hepatic kynurenine pathway and immune surveillance

Extended Data Fig. 8: Qprt overexpression promotes hepatic CD8+ T cell homeostasis.The alternative text for this image may have been generated using AI.

(A-C) Control and CUMS mice were injected with vector or AAV8-TBG-Qprt (n = 6 mice per group). Western blot and RT-qPCR analysis of Qprt expression in hepatocytes (A), FACS plots and graphs for the number (B) and the expression of exhaustion markers (C) in liver CD8+T cells. (D-I) Purified OT-1-CD8+T cells were co-cultured with Adrb2f/f and Adrb2Δhep hepatocytes infected with empty or Qprt-overexpressing lentivirus (n = 3 biologically independent samples). (D) Experimental schemes, (E) Western blot analysis of QPRT expression in hepatocytes. (F) ELISA determination of NAD+ and KA levels hepatocytes. (G) CD8+T cells killing activity against EL-4 cells. FACS analysis of apoptosis (H) and IFN-γ, TNF, GZMB expression (I) in CD8+T cells. Dots represent data from individual mice or different cell replicates, and error bars indicate as the mean ± s.e.m. in one experiment. Statistical analyses were done by one-way ANOVA with Sidak’s multiple comparisons test (A, B, C, F, H, I) or Two-way ANOVA with Sidak’s multiple comparisons test (G). Illustration in D created in BioRender.com.

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