Extended Data Fig. 9: NAM and KA inhibitors restore mitochondrial homeostasis in hepatic CD8+ T cells. | Nature Metabolism

Extended Data Fig. 9: NAM and KA inhibitors restore mitochondrial homeostasis in hepatic CD8+ T cells.

From: Chronic stress drives liver cancer by impairing the hepatic kynurenine pathway and immune surveillance

Extended Data Fig. 9: NAM and KA inhibitors restore mitochondrial homeostasis in hepatic CD8+ T cells.The alternative text for this image may have been generated using AI.

(A) FACS analysis of dysfunctional mitochondrial in liver CD8+T cells cocultured with Adrb2f/f and Adrb2Δhep hepatocytes infected with Lv-vector or Lv-Qprt (n = 3 biologically independent samples). (B-C) Liver CD8+T cells co-cultured with Adrb2f/f and Adrb2Δhep hepatocytes were treated with or without NAM (20 mM) and CH223191 (10 μM). (B) FACS analysis of dysfunctional mitochondrial in co-cultured CD8+T cells (n = 6 biologically independent samples). (C) Representative images for oxygen OCR and the basal respiration, maximum respiration, spare respiratory capacity and ATP production were analyzed in co-cultured CD8+T cells (n = 4 biologically independent samples). Dots represent data from different cell replicates, and error bars indicate as the mean ± s.e.m. in one experiment. Statistical analyses were done by one-way ANOVA with Sidak’s multiple comparisons test.

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