Extended Data Fig. 7: Glucose utilisation in cachexia target tissues.
(a-e) Tracing experiment using isotopically labelled glucose ([13C6]-glucose) in healthy controls (Ctrl), non-cachectic (Non-cax), pre-cachectic (Pre-cax) and cachectic (Cax) mice with tumours. n = 4 animals per group. See also Fig. 1a for experimental setup. Incorporation of labelled carbons from [13C6]-glucose into metabolites of the TCA cycle in plasma (a), liver (b), eWAT (c), iWAT (d), and tumour (e). Unlabelled metabolites are referred as M + 0, isotopically labelled metabolites as M + X, where X represent the number of labelled carbon atoms. Data are presented as MS signal intensity (arbitrary units A.U.). Data are mean ± s.e.m. Statistical analysis: two-way ANOVA with Dunnett’s post-hoc tests vs. Cax. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to Cax.
